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Parenteral B12 use: Deficiency of B12 causes megaloblastic anemia and neurological symptoms (fatigue, tingling, memory problems)

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This pathway generates pentose sugar for amino acid and nucleic acid synthesis, converting glucose to ribulose-5-phosphate via the pentose phosphate shunt

Cells were extensively washed with PBS, fixed in 4% PFA for 15 min, permeabilized and blocked with 0.01% saponin/10% normal goat/PBS serum and stained with the primary antibodies in the same blocking solution in a humid chamber

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